Workshops

This workshop will focus on protein-ligand interactions, including both protein- and ligand-based methods for identifying, quantifying and mapping binding interactions using solution NMR. It includes both lectures and hands-on sessions at the spectrometer for data acquisition and at the computer for data analysis.

Topics include:

•Introduction to NMRFAM, NMRbox and the Network for Advanced NMR.

•Introduction to NMR of proteins and small molecule ligands

•Hands-on solution NMR data acquisition of solution NMR data

•Data processing, including uniformly- and non-uniformly sampled data

•Peak detection and quantification for chemical shift perturbation and ligand screening

•Using TREND NMR to analyze protein-ligand binding

•Hands-on backbone assignment with automated methods and manual verification

•Mapping chemical shift perturbations

•Fragment-based drug-discovery, design of screening workflows and practical applications

Bring a poster describing your project or research goals for the poster session on Monday 7/27/26.

Contact us if you would like more individualized consultation on a specific project on Friday 7/31/26!

Presenters include:

Katie Henzler-Wildman, Marco Tonelli, Paulo Falco-Cobra (NMRFAM, UW-Madison);

Brian Volkman, Paul Kerber (Medical College of Wisconsin),

Steve Van Doren (University of Missouri),

Jeff Hoch, Mark Maciejewski (UConn Health), and

Frank Delaglio (University of Maryland/NIST)

First come first serve basis, with a limit of up to 25 participants.

Lodging Options.

Questions? Email Lai Bergeman.

Workshop contents and recordings are available to registered participants at NMRbox.

This workshop covered important considerations in sample preparation and experimental design for protein solution NMR with a focus on ILV-methyl labeled protein experiments. It includes both lectures and hands-on session at the spectrometer for data acquisition and at the computer for data analysis. Presenters include Frank Delaglio, Marco Tonelli, Mike Latham, Art Edison, and Katie Henzler-Wildman.

• Introduction to the Network for Advanced NMR.
• What can NMR do for you? What is NMR good for in the modern era of structural biology?
• Hands-on solution NMR data acquisition of 2D and 3D NMR data for proteins. Focus on experiments studies of U-¹⁵N,²H, ¹H¹³C-methyl-ILV- labeled proteins: ¹H¹⁵N HSQC, ¹H¹⁵N TROSY, ¹H¹³C HMQC, 3D methyl-NOESY-HMQC.
• Data processing, including uniformly- and non-uniformly sampled data with nmrPipe, presented by Frank Delaglio.
• Hands-on structure-based assignment of ILV methyl-labeled proteins using NOESY data and RDCs.
• Introduction to protein methyl dynamics. Practical aspects of data acquisition, processing, and data analysis for MQ-CPMG, methyl ¹H-triple quantum “forbidden” experiments.
• Poster session – share your project and how you are using or planning to use NMR. Network with other participants and get feedback from expert NMR staff.

Solution NMR Structural Biology Workshop 

June 5-9, 2023 at UGA

Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)

Instructors include:

Frank Delaglio (Institute for Bioscience and Biotechnology Research/NIST)

Art Edison, Jim Prestegard, John Glushka, and Alex Eletsky (University of Georgia)

Sam Butcher, Riley Petersen, Marco Tonelli, and Katie Henzler-Wildman (University of Wisconsin-Madison)

Mark Maciejewski (University of Connecticut Health Center)

Solution NMR Structural Biology Workshop – We had a successful Workshop! See you in Georgia next year!
June 20-24, 2022 at NMRFAM
Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)
Presenters include Katie Henzler-Wildman, Marco Tonelli, Vilius Kurauskas (NMRFAM) and Art Edison, Jim Prestegard, Alex Eletsky (CCRC), and Jeff Hoch (UConn)

2022 Solution NMR Topics

Virtual Workshop – Access Anytime!
Presented June 2021
Step by step training in how to assign your protein using NMRFAM-Sparky

Manual Backbone Assignments – Szymon Ciesielski
Q&A video 1
Q&A video 2

Manual Sidechain Assignments – Marco Tonelli
Q&A video 1
Q&A video 2
Updated side chain assignment folder

APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
Q&A video

I-PINE – Kumaran Baskaran
Q&A video

Virtual Workshop – Access Anytime!
Presented June 2020
Step by step training in how to assign your protein using NMRFAM-Sparky

NMRFAM Protein Structure Determination Workshop – Milo Westler

NMRFAM SPARKY – Woonghee Lee

Manual Backbone Assignments – Szymon Ciesielski

Manual Sidechain Assignments – Marco Tonelli

APES(Automated Peak Picking of Spectra)  – Mehdi Rahimi

I-PINE  – Kumaran Baskaran

BMRB Overview and data deposition  – Kumaran Baskaran