Workshops

This workshop will cover important considerations in sample preparation and experimental design for protein solution NMR and provide hands-on training in assigning resonances to specific nuclei in the protein for site-specific interpretation of NMR data and analysis of chemical shift perturbations.

Topics Include:

• Introduction to the new Network for Advanced NMR.
• What can NMR do for you? What is NMR good for in the modern era of structural biology?
• Overview of NMR workflows – where do you start and what are the steps to take if you’re interested in structure, dynamics, molecular interactions or other applications of NMR?
• Hands-on assignment of the protein backbone. Amide HSQC or TROSY spectra are the standard fingerprint spectrum in protein NMR. Backbone assignment is the first step in structure determination, and amide resonance assignments are commonly used to map site-specific ligand interactions or monitor protein dynamics with site-specific resolution. Automated and manual methods
• Hands-on structure-based assignment of ILV methyl-labeled proteins using NOESY data. Methyl groups provide better sensitivity and resolution for larger systems, and are used to map site-specific ligand interactions or monitor protein dynamics with site-specific resolution. This process also provides experimental validation of your structural model (homology model, structure prediction from Alpha Fold, etc.).
• Assignment of sparsely-labeled eukaryotic proteins. Isotopic labeling of a reduced set of amino acids provides a cost-effective route to NMR studies of proteins that are produced in eukaryotic cells, including glycoproteins. Assignment of these sparse labels is also structure-based using NOESY, RDC, PCS and PRE data, and provides markers that can be used to monitor structure, dynamics, and interactions in such systems.
• Introduction to mapping ligand-protein and protein-protein interactions with chemical shift and paramagnetic perturbations.
• Poster session – share your project and how you are using or planning to use NMR. Network with other participants and get feedback from expert NMR staff.

Schedule and Important Dates

March and April, 2022 – Check our website for workshop dates, format, and registration information. The cost of this workshop is $100.00. The deadline for registration is June 6th, 2022 and will be on first come, first serve basis.  Lodging options for the workshop can be found here.

Please send registration questions to lai.bergeman@wisc.edu.

We hope you can participate in this popular workshop and become an NMRFAM user!

Funding is available to support workshop attendance, including the cost of registration, housing, and potentially travel. Please send your CV and a short statement of need to Lai Bergeman [lai.bergeman@wisc.edu]. URM students from minority-serving or undergraduate institutions are especially encouraged to apply.

Workshop participants are not expected to have prior experience with basic steps of NMR protein assignments. The workshop will cover the entire process and each session will build upon the previous sessions. Participants will be given the data necessary to complete each session.

Computer Requirements: In order to install VirtualBox, you MUST have administrative privileges on your computer. It is recommended that the computer have at least 4 GB of memory. The memory and bit size are not strict requirements, but the speed will suffer. The computer should have ~10 GB of free disk space. It is recommended that you have a (preferably 2-button with scroll wheel) mouse for your computer.

This workshop will provide hands-on experience to beginning to intermediate solid-state NMR users, and is designed to complement the theoretical introduction to solid-state NMR provided by the Solid-State NMR Winter School (https://winterschool.mit.edu/). Topics include:

• Hands on practice in packing and spinning rotors using rotor-packing devices, including both powder and sticky biological samples.
• Hands on practice in probe configuration and tuning, of particular interest for materials science applications.
• Hands-on practice in writing/modifying, compiling, and testing pulse sequences (Bruker Topspin).
• Hands-on practice in experimental setup and data acquisition, including optimization of cross-polarization, decoupling, and other key parameters.

Schedule and Important Dates

May and June, 2022 – Check our website for workshop dates, format, and registration information. Registration will be on first come, first serve basis.

Please send registration questions to lai.bergeman@wisc.edu

Workshop participants are not expected to have prior experience with basic steps of NMR protein assignments. The workshop will cover the entire process and each session will build upon the previous sessions. Participants will be given the data necessary to complete each session.

Computer Requirements: In order to install VirtualBox, you MUST have administrative privileges on your computer. It is recommended that the computer have at least 4 GB of memory. The memory and bit size are not strict requirements, but the speed will suffer. The computer should have ~10 GB of free disk space. It is recommended that you have a (preferably 2-button with scroll wheel) mouse for your computer.

Virtual Workshop – Access Anytime!
Presented June 2021
Step by step training in how to assign your protein using NMRFAM-Sparky

Manual Backbone Assignments – Szymon Ciesielski
Q&A video 1
Q&A video 2

Manual Sidechain Assignments – Marco Tonelli
Q&A video 1
Q&A video 2
Updated side chain assignment folder

APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
Q&A video

I-PINE – Kumaran Baskaran
Q&A video

Virtual Workshop – Access Anytime!
Presented June 2020
Step by step training in how to assign your protein using NMRFAM-Sparky

NMRFAM Protein Structure Determination Workshop – Milo Westler

NMRFAM SPARKY – Woonghee Lee

Manual Backbone Assignments – Szymon Ciesielski

Manual Sidechain Assignments – Marco Tonelli

APES(Automated Peak Picking of Spectra)  – Mehdi Rahimi

I-PINE  – Kumaran Baskaran

BMRB Overview and data deposition  – Kumaran Baskaran

Coming to an NMRFAM near you soon!