Workshops

Schedule and Important Dates 

Registration will be on first come, first serve basis. The cost of this workshop is $200.00. Payment is required before workshop and is NON REFUNDABLE. The deadline for registration is July 4, 2025.

Please submit Registration form and any questions to Lai Bergeman (lai.bergeman@wisc.edu)

Lodging options for the workshop can be found here.

This workshop will provide hands-on experience to beginning to intermediate solid-state NMR users, and is designed to complement the theoretical introduction to solid-state NMR provided by the Solid-State NMR Winter School (https://winterschool.mit.edu/). Topics include:

• Hands on practice in packing and spinning rotors using rotor-packing devices, including both powder and sticky biological samples.
• Hands on practice in probe configuration and tuning, of particular interest for materials science applications.
• Hands-on practice in measuring pulse sequence timing, power and phase shifts, and modifying pulse sequences (Bruker Topspin).
• Hands-on practice in experimental setup and data acquisition, including optimization of cross-polarization, decoupling, and other key parameters.

Solution NMR Structural Biology Workshop

Virtual Workshop, August 11-15, 2025

Presented by the NMRFAM, UW-Madison

This workshop will cover fundamentals of protein assignment.

Topics include:

• Introduction to the NMRFAM and the Network for Advanced NMR
• Hands-on: How to use NMRbox and NMRFAM-Sparky
• Overview of protein backbone assignment
• Hands-on: manual backbone assignment in NMRFAM-Sparky
• Hands-on: automated backbone assignment with PINE
• Overview: backbone assignment for larger proteins, strategies for challenging systems
• Overview: methyl assignment
• Hands-on: methyl assignment, linking to backbone
• Hands-on: methyl assignment, structure-based approach

Please submit Registration form and any questions to Lai Bergeman (lai.bergeman@wisc.edu)

** You will need an NMRhub account to access workshop materials. Please create one as soon as possible if you do not already have one. **

This workshop covered important considerations in sample preparation and experimental design for protein solution NMR with a focus on ILV-methyl labeled protein experiments. It includes both lectures and hands-on session at the spectrometer for data acquisition and at the computer for data analysis. Presenters include Frank Delaglio, Marco Tonelli, Mike Latham, Art Edison, and Katie Henzler-Wildman.

• Introduction to the Network for Advanced NMR.
• What can NMR do for you? What is NMR good for in the modern era of structural biology?
• Hands-on solution NMR data acquisition of 2D and 3D NMR data for proteins. Focus on experiments studies of U-¹⁵N,²H, ¹H¹³C-methyl-ILV- labeled proteins: ¹H¹⁵N HSQC, ¹H¹⁵N TROSY, ¹H¹³C HMQC, 3D methyl-NOESY-HMQC.
• Data processing, including uniformly- and non-uniformly sampled data with nmrPipe, presented by Frank Delaglio.
• Hands-on structure-based assignment of ILV methyl-labeled proteins using NOESY data and RDCs.
• Introduction to protein methyl dynamics. Practical aspects of data acquisition, processing, and data analysis for MQ-CPMG, methyl ¹H-triple quantum “forbidden” experiments.
• Poster session – share your project and how you are using or planning to use NMR. Network with other participants and get feedback from expert NMR staff.

Solution NMR Structural Biology Workshop 

June 5-9, 2022 at UGA

Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)

Instructors include:

Frank Delaglio (Institute for Bioscience and Biotechnology Research/NIST)

Art Edison, Jim Prestegard, John Glushka, and Alex Eletsky (University of Georgia)

Sam Butcher, Riley Petersen, Marco Tonelli, and Katie Henzler-Wildman (University of Wisconsin-Madison)

Mark Maciejewski (University of Connecticut Health Center)

Solution NMR Structural Biology Workshop – We had a successful Workshop! See you in Georgia next year!
June 20-24, 2022 at NMRFAM
Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)
Presenters include Katie Henzler-Wildman, Marco Tonelli, Vilius Kurauskas (NMRFAM) and Art Edison, Jim Prestegard, Alex Eletsky (CCRC), and Jeff Hoch (UConn)

2022 Solution NMR Topics

Virtual Workshop – Access Anytime!
Presented June 2021
Step by step training in how to assign your protein using NMRFAM-Sparky

Manual Backbone Assignments – Szymon Ciesielski
Q&A video 1
Q&A video 2

Manual Sidechain Assignments – Marco Tonelli
Q&A video 1
Q&A video 2
Updated side chain assignment folder

APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
Q&A video

I-PINE – Kumaran Baskaran
Q&A video

Virtual Workshop – Access Anytime!
Presented June 2020
Step by step training in how to assign your protein using NMRFAM-Sparky

NMRFAM Protein Structure Determination Workshop – Milo Westler

NMRFAM SPARKY – Woonghee Lee

Manual Backbone Assignments – Szymon Ciesielski

Manual Sidechain Assignments – Marco Tonelli

APES(Automated Peak Picking of Spectra)  – Mehdi Rahimi

I-PINE  – Kumaran Baskaran

BMRB Overview and data deposition  – Kumaran Baskaran