Training
NMRFAM has a long history of providing workshops to train beginning users and non-experts in NMR data analysis. We are developing new content, including modern solution NMR data analysis, sample preparation, and hands-on training in solid-state NMR rotor packing, spectrometer setup and data acquisition.
2025 Solid-State NMR Hands-On Workshop August 5-8, 2025 (In-person workshop)
Hosted by NMRFAM at UW Madison
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2025 Registration Information
Schedule and Important Dates
Registration will be on first come, first serve basis. The cost of this workshop is $200.00. Payment is required before workshop and is NON REFUNDABLE. The deadline for registration is July 4, 2025.
Please submit Registration form and any questions to Lai Bergeman (lai.bergeman@wisc.edu)
Lodging options for the workshop can be found here.
Workshop Contents
This workshop will provide hands-on experience to beginning to intermediate solid-state NMR users, and is designed to complement the theoretical introduction to solid-state NMR provided by the Solid-State NMR Winter School (https://winterschool.mit.edu/). Topics include:
- Hands on practice in packing and spinning rotors using rotor-packing devices, including both powder and sticky biological samples.
- Hands on practice in probe configuration and tuning, of particular interest for materials science applications.
- Hands-on practice in measuring pulse sequence timing, power and phase shifts, and modifying pulse sequences (Bruker Topspin).
- Hands-on practice in experimental setup and data acquisition, including optimization of cross-polarization, decoupling, and other key parameters.
Participant Requirements
2025 Solution NMR Structural Biology Workshop August 11-15,2025 (Virtual Workshop)
Presented by NMRFAM (UW-Madison)
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2025 Registration Information
Solution NMR Structural Biology Workshop
Virtual Workshop, August 11-15, 2025
Presented by the NMRFAM, UW-Madison
This workshop will cover fundamentals of protein assignment.
Topics include:
- Introduction to the NMRFAM and the Network for Advanced NMR
- Hands-on: How to use NMRbox and NMRFAM-Sparky
- Overview of protein backbone assignment
- Hands-on: manual backbone assignment in NMRFAM-Sparky
- Hands-on: automated backbone assignment with PINE
- Overview: backbone assignment for larger proteins, strategies for challenging systems
- Overview: methyl assignment
- Hands-on: methyl assignment, linking to backbone
- Hands-on: methyl assignment, structure-based approach
Please submit Registration form and any questions to Lai Bergeman (lai.bergeman@wisc.edu)
** You will need an NMRhub account to access workshop materials. Please create one as soon as possible if you do not already have one. **
Past Solution Workshops
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2024 Solution NMR Structural Biology Workshop June 4-7, 2024 at NMRFAM (UW-Madison)
This workshop covered important considerations in sample preparation and experimental design for protein solution NMR with a focus on ILV-methyl labeled protein experiments. It includes both lectures and hands-on session at the spectrometer for data acquisition and at the computer for data analysis. Presenters include Frank Delaglio, Marco Tonelli, Mike Latham, Art Edison, and Katie Henzler-Wildman.
- Introduction to the Network for Advanced NMR.
- What can NMR do for you? What is NMR good for in the modern era of structural biology?
- Hands-on solution NMR data acquisition of 2D and 3D NMR data for proteins. Focus on experiments studies of U-15N,2H, 1H13C-methyl-ILV- labeled proteins: 1H15N HSQC, 1H15N TROSY, 1H13C HMQC, 3D methyl-NOESY-HMQC.
- Data processing, including uniformly- and non-uniformly sampled data with nmrPipe, presented by Frank Delaglio.
- Hands-on structure-based assignment of ILV methyl-labeled proteins using NOESY data and RDCs.
- Introduction to protein methyl dynamics. Practical aspects of data acquisition, processing, and data analysis for MQ-CPMG, methyl 1H-triple quantum “forbidden” experiments.
- Poster session – share your project and how you are using or planning to use NMR. Network with other participants and get feedback from expert NMR staff.
2023 Solution NMR Structural Biology Workshop June 5-9 , 2023 at CCRC NMR Facility, University of Georgia (UGA)
Solution NMR Structural Biology Workshop
June 5-9, 2022 at UGA
Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)
Instructors include:
Frank Delaglio (Institute for Bioscience and Biotechnology Research/NIST)
Art Edison, Jim Prestegard, John Glushka, and Alex Eletsky (University of Georgia)
Sam Butcher, Riley Petersen, Marco Tonelli, and Katie Henzler-Wildman (University of Wisconsin-Madison)
Mark Maciejewski (University of Connecticut Health Center)
2022 NMRFAM Solution NMR Structural Biology Workshop - June 20-24, 2022
Solution NMR Structural Biology Workshop – We had a successful Workshop! See you in Georgia next year!
June 20-24, 2022 at NMRFAM
Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)
Presenters include Katie Henzler-Wildman, Marco Tonelli, Vilius Kurauskas (NMRFAM) and Art Edison, Jim Prestegard, Alex Eletsky (CCRC), and Jeff Hoch (UConn)
2021 NMRFAM Virtual Introductory Structure Determination Workshop Q&A sessions
Virtual Workshop – Access Anytime!
Presented June 2021
Step by step training in how to assign your protein using NMRFAM-Sparky
Manual Backbone Assignments – Szymon Ciesielski
Q&A video 1
Q&A video 2
Manual Sidechain Assignments – Marco Tonelli
Q&A video 1
Q&A video 2
Updated side chain assignment folder
APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
Q&A video
I-PINE – Kumaran Baskaran
Q&A video
2020 NMRFAM Virtual Introductory Structure Determination Workshop
Virtual Workshop – Access Anytime!
Presented June 2020
Step by step training in how to assign your protein using NMRFAM-Sparky
NMRFAM Protein Structure Determination Workshop – Milo Westler
NMRFAM SPARKY – Woonghee Lee
Manual Backbone Assignments – Szymon Ciesielski
Manual Sidechain Assignments – Marco Tonelli
APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
I-PINE – Kumaran Baskaran
BMRB Overview and data deposition – Kumaran Baskaran
Past Solid State NMR Workshops
2024 Solid-State NMR Hands-On Workshop July23-26, 2024 at NMRFAM
2023 Solid-State NMR Hands-On Workshop Aug 1-4th, 2023 at NMRFAM
2022 Solid-State NMR Hands-On Workshop – October 17-20, 2022 at NMRFAM