National Magnetic Resonance Facility at Madison

Workshops

Training

NMRFAM has a long history of providing workshops to train beginning users and non-experts in NMR data analysis. We are developing new content, including modern solution NMR data analysis, sample preparation, and hands-on training in solid-state NMR rotor packing, spectrometer setup and data acquisition.

 

2024 Solution NMR Structural Biology Workshop  June 4-7 , 2024 at NMRFAM (We had a successful and full workshop! See you next year!)

Presented jointly by NMRFAM (UW-Madison), University of Minnesota NMR Center (UMN), and CCRC NMR Facility (UGA)

This is an accordion element with a series of buttons that open and close related content panels.

2024 Scheduling Information (Registration is CLOSED)

Schedule and Important Dates 

Registration will be on first come, first serve basis. The cost of this workshop is $200.00. The deadline for registration is May 21, 2024 and will be on first come, first serve basis.

Please submit Registration form and any questions to Lai Bergeman (lai.bergeman@wisc.edu)

Lodging options for the workshop can be found here.

Workshop Contents

This workshop will cover important considerations in sample preparation and experimental design for protein solution NMR with a focus on ILV-methyl labeled protein experiments. It includes both lectures and hands-on session at the spectrometer for data acquisition and at the computer for data analysis. Presenters include Frank Delaglio, Marco Tonelli, Mike Latham, Art Edison, and Katie Henzler-Wildman.

  • Introduction to the Network for Advanced NMR.
  • What can NMR do for you? What is NMR good for in the modern era of structural biology?
  • Hands-on solution NMR data acquisition of 2D and 3D NMR data for proteins. Focus on experiments studies of U-15N,2H, 1H13C-methyl-ILV- labeled proteins: 1H15N HSQC, 1H15N TROSY, 1H13C HMQC, 3D methyl-NOESY-HMQC.
  • Data processing, including uniformly- and non-uniformly sampled data with nmrPipe, presented by Frank Delaglio.
  • Hands-on structure-based assignment of ILV methyl-labeled proteins using NOESY data and RDCs.
  • Introduction to protein methyl dynamics. Practical aspects of data acquisition, processing, and data analysis for MQ-CPMG, methyl 1H-triple quantum “forbidden” experiments.
  • Poster session – share your project and how you are using or planning to use NMR. Network with other participants and get feedback from expert NMR staff.

 

2024 Solid-State NMR Hands-On Workshop July 23-26, 2024 (We had a successful and full workshop! See you next year!)

Hosted by NMRFAM at UW Madison 

This is an accordion element with a series of buttons that open and close related content panels.

2024 Scheduling Information (Registration is CLOSED)

Schedule and Important Dates 

Registration will be on first come, first serve basis. The cost of this workshop is $200.00. The deadline for registration is June 1, 2024.

Please submit Registration form and any questions to Lai Bergeman (lai.bergeman@wisc.edu)

Lodging options for the workshop can be found here.

Workshop Contents

This workshop will provide hands-on experience to beginning to intermediate solid-state NMR users, and is designed to complement the theoretical introduction to solid-state NMR provided by the Solid-State NMR Winter School (https://winterschool.mit.edu/). Topics include:

  • Hands on practice in packing and spinning rotors using rotor-packing devices, including both powder and sticky biological samples.
  • Hands on practice in probe configuration and tuning, of particular interest for materials science applications.
  • Hands-on practice in measuring pulse sequence timing, power and phase shifts, and modifying pulse sequences (Bruker Topspin).
  • Hands-on practice in experimental setup and data acquisition, including optimization of cross-polarization, decoupling, and other key parameters.

Participant Requirements

Workshop participants who have some prior introductory experience with solid-state NMR will benefit most from this workshop, although we are also happy to use this forum as a means to introduce new users to the magic of spinning and the power of decoupling.  Workshop content will primarily focus on biosolids NMR, but users interested in materials applications are also very welcome!  Many of the methods, especially the instrumentation aspects, are generally applicable to both biosolids and materials, and NMRFAM is developing a major new focus in materials solid-state NMR and looking for collaborators and users.
 
All workshop participants will have the opportunity to do “hands on” work with rotor packing and spinning, probe tuning, pulse sequence editing and compiling, and configuration/optimization of experiments at NMRFAM spectrometers. 
 
Along these lines, NMRFAM will provide standard samples to participants, but we will also be glad to support workshop participants who wish to bring their own research samples along with them to the workshop.  This is very much encouraged, and in order to coordinate this in advance we would like participants to let us know by Thursday, July 11, 2024 if they are interested in bringing along their own research samples. We will then use this information to coordinate individual work plans for the week of the workshop. In cases where we run out of time at spectrometers to collect the data during the workshop for everyone who is interested, data collection will continue after the workshop with support of NMRFAM staff and the data shared with the participants remotely.  Of course, we also encourage participants to visit NMRFAM in the future to continue these projects.
 
In addition, please let us know if you have exotic nuclei of interest and/or special requests for pulse sequences that you wish to use in your research.  We will primarily use our standard NMRFAM (and Bruker) pulse sequence libraries, but if given sufficient advanced notice, we can consider implementation of other pulse sequences that you wish to use in your research. Again, if we are not able to complete this during the workshop itself, NMRFAM staff will follow up in collaboration with you afterwards to help solve your research problems.
 
Finally, please also let us know in advance if you require accommodations of any type and we will make every effort to prepare for your visit.

Past Solution Workshops

This is an accordion element with a series of buttons that open and close related content panels.

2023 Solution NMR Structural Biology Workshop  June 5-9 , 2023 at CCRC NMR Facility, University of Georgia (UGA) 

Solution NMR Structural Biology Workshop 

June 5-9, 2022 at UGA

Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)

Instructors include:

Frank Delaglio (Institute for Bioscience and Biotechnology Research/NIST)

Art Edison, Jim Prestegard, John Glushka, and Alex Eletsky (University of Georgia)

Sam Butcher, Riley Petersen, Marco Tonelli, and Katie Henzler-Wildman (University of Wisconsin-Madison)

Mark Maciejewski (University of Connecticut Health Center)

2022 NMRFAM Solution NMR Structural Biology Workshop - June 20-24, 2022

Solution NMR Structural Biology Workshop – We had a successful Workshop! See you in Georgia next year!
June 20-24, 2022 at NMRFAM
Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)
Presenters include Katie Henzler-Wildman, Marco Tonelli, Vilius Kurauskas (NMRFAM) and Art Edison, Jim Prestegard, Alex Eletsky (CCRC), and Jeff Hoch (UConn)

2022 Solution NMR Topics

2021 NMRFAM Virtual Introductory Structure Determination Workshop Q&A sessions

Virtual Workshop – Access Anytime!
Presented June 2021
Step by step training in how to assign your protein using NMRFAM-Sparky

Manual Backbone Assignments – Szymon Ciesielski
Q&A video 1
Q&A video 2

Manual Sidechain Assignments – Marco Tonelli
Q&A video 1
Q&A video 2
Updated side chain assignment folder

APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
Q&A video

I-PINE – Kumaran Baskaran
Q&A video

2020 NMRFAM Virtual Introductory Structure Determination Workshop

Virtual Workshop – Access Anytime!
Presented June 2020
Step by step training in how to assign your protein using NMRFAM-Sparky

NMRFAM Protein Structure Determination Workshop – Milo Westler

NMRFAM SPARKY – Woonghee Lee

Manual Backbone Assignments – Szymon Ciesielski

Manual Sidechain Assignments – Marco Tonelli

APES(Automated Peak Picking of Spectra)  – Mehdi Rahimi

I-PINE  – Kumaran Baskaran

BMRB Overview and data deposition  – Kumaran Baskaran

Past Solid State NMR Workshops

2023 Solid-State NMR Hands-On Workshop Aug 1-4th, 2023 at NMRFAM

2022 Solid-State NMR Hands-On Workshop – October 17-20, 2022 at NMRFAM