National Magnetic Resonance Facility at Madison

e-Newsletter

May 2026

NEWS & UPDATES

  • NMRFAM will be shutdown from 5/18-5/21 for maintenance.
  • NMRFAM is not a walk-in facility. Users must schedule spectrometer time request through iLab. Approval of time requests will be sent to users by email via iLab.
  • NMRFAM will host the Solution Workshop on July 27-31. This year’s hands-on workshop will focus on protein assignment and ligand interaction. Stay tuned – workshop details and registration form will be available at our website starting May 20.

NEW USER INFORMATION

  • Lakenvelder, the 1.1 GHz solid-state NMR (SSNMR) Bruker NEO spectrometer located at NMRFAM, has been fully installed and is available for community access. We are excited to collaborate with new users and help them leverage this unique resource. To request access, please submit your application through the NAN portal at https://usnan.nmrhub.org/uhf-access-instructions.
  • NMRFAM provides access to high and ultra high field (500-1100 MHz) solution and solid-state NMR instrumentation and a team of expert staff to assist researchers in experimental design, data acquisition or data analysis. Please contact us with your project ideas and spectrometer needs. Please visit the NMRFAM website for more information.
  • Solution NMR spectrometers (Fleckvieh 900 MHz, Gelbvieh 800 MHz, Telemark 750 MHz, Kurgan 600 MHz, Dexter 600 MHz, Kerry 500 MHz) are all equipped with 5 mm triple resonance HCN cryoprobes. Additional HPCN and HFCN quad cryoprobes are available at 600 MHz. A 1.7mm cryoprobe is also available at 600 MHz. SampleJet with capacity for up to 500 samples is available at 500 MHz and 600 MHz.
  • Varian SSNMR spectrometers (Ayrshire 750wb, Taurus 600, Ribeye 600) feature a customized version of OpenVNMRJ 3.2 with advanced OPTO automation capabilities. These systems are optimized for 1.6 mm and 3.2 mm Varian and Phoenix probes, including HCN, HFCN, HPCN and other broadband configurations.
  • Bruker SSNMR spectrometers (Lakenvelder 1.1 GHz, Girolando 900 MHz, Vosges 600 MHz) offer unique performance with emphasis on high performance 13C detection at high and ultra-high field.
  • For a full comparison of 13C detection sensitivity among the NMRFAM solid probes, please see Han et al. JMR 2024: https://doi.org/10.1016/j.jmr.2024.107709
  • NMRFAM is part of the Network for Advanced NMR (NAN). If you’d like to know more about NAN, please see this recent publication:
    Scalable cyberinfrastructure for experimental NMR data. Hoch JC, Henzler-Wildman K, Edison AS, Rienstra CM, Bontempi C, Wedell JR, Weatherby G, Burr H, Pustovalova Y, Thongdee S, Gryk MR, Pozhidaeva A, Simon B, Cheng Q, Wilson MP, Moraru II, Morris L, Glushka JN, Uchimiya M, Eletsky A, Moore AE, Grimes JH Jr, Paterson AL, Wang S, Pinheiro PR, Vanderloop BH, Maciejewski MW. Sci Data. 2025 Dec.
  • Our Solution and Solid spectrometer capabilities.

Latest publications:

  1. Probing Solution Dynamics of Tissue Factor Using Molecular Dynamics Simulations Guided by NMR Chemical Shifts. Lihan M, Pant S, Ojoawo AM, Rienstra CM, Tajkhorshid E. J Phys Chem B. 2026 Marc. PMID:4189586.
  2. In vitro-prepared A30P alpha-synuclein fibrils adopt the conserved and disease-relevant Greek key fold. Milchberg MH, Warmuth OA, Borcik CG, Han R, Harding BD, Dhavale DD, Kotzbauer PT, Wright ER, Schwieters CD, Rienstra CM. bioRxiv (preprint) 2026 Feb. PMID:41676702.
  3. A structural model of toxic amyloid oligomers involved in type 2 diabetes. Shivani ST, LeMasters BE, Ravula T, Esterly HJ, Maroli N, Rich KL, Fields CR, Dicke SS, Warmuth OA, Stapleton DS, Keller MP, Attie AD, Kananenka AA, Henzler-Wildman KA, Rienstra CM, Zanni MT. Proc Natl Acad Sci USA. 2026 Feb. PMID:41587315.
  4. Impact of shared facilities in advancing solid-state NMR research: 2025 edition. Schurko RW, Rienstra CM, Jaroniec CP, Hansen AL, Franks WT, Bryce DL, Brinkmann A, Terskikh V, Brown SP, Iuga D, van Heijenoort C, Fayon F, Bertaina S, Alfonso C, Karlsson G, Grobner G, Potzebowski MJ, Cerofolini L, Ravera E, Fragai M, Lelli M, Lesage A, Pintacuda G, Pons M, Mafra L, Schneider JF, Monti GA, Acosta RH, Pastawski HM, Thomas B, Kolyagin YG, Agarwal V, Hou G, Deng F, Xue K, Kigawa T, Manjunatha Reddy GN. Solid State Nuclear Magn Reson. 2026 Feb. PMID:4142257.
  5. Top-Down Scoring of Spectral Fitness by Image Analysis for Protein Structure Validation. Harding BD, Dezonia B, Gang R, Hu Z, Delaglio F, Grant T, Rienstra CM. J Chem Inf Model. 2026 Jan. PMID:41417269.
  6. Multidrug Resistance Transporter EmrE is Only Moderately Sensitive to Lipid Composition. Hiett AB, Hisao GS, Robinson AE, Morrison EA, Henzler-Wildman KA. Biophys J. 2026 Jan. PMID:41612699.
  7. Host-mirobiome mutualism drives urea carbon salvage and acetogenesis during hibernation. Regan MD, Chiang E, Grahn M, Tenelli M, Assadi-Porter FM, Suen G, Carey HV. Proc Natl Acad Sci USA. 2026 Jan. PMID:41481471.
  9. Energetic and structural control of polyspecificity in a multidrug transporter. Miller ST, Henzler-Wildman KA, Raman S. Proc Natl Acad Sci USA. 2025 Dec. PMID:41433063.
  10. Scalable cyberinfrastructure for experimental NMR data. Hoch JC, Henzler-Wildman K, Edison AS, Rienstra CM, Bontempi C, Wedell JR, Weatherby G, Burr H, Pustovalova Y, Thongdee S, Gryk MR, Pozhidaeva A, Simon B, Cheng Q, Wilson MP, Moraru II, Morris L, Glushka JN, Uchimiya M, Eletsky A, Moore AE, Grimes JH Jr, Paterson AL, Wang S, Pinheiro PR, Vanderloop BH, Maciejewski MW. Sci Data. 2025 Dec.
  11. NMR Spectral Alignment Utilizing a CryoEM Motion Correction Algorithm. Hemme CA, Warmuth OA, Wang S, Williams CG, Thorne A, Mueller LJ, Rienstra CM, Grant T. Anal Chem. 2025 Dec. PMID:41396659.
  12. Selective Detection of a Key Region in Chemotaxis Signaling Protein Complexes by Solid-State NMR. Allen JJ, Wang S, Rienstra CM, Thompson LK. Biophys J. 2025 Sept. PMID:41017145.
  13. Actinomycetoquinones A-E, Anthraquinone-y-Pyrones Discovered from Marine-Derived Actinomycetospora sp. Bacterium. Zhang F, Roberts CD, Lee TH, Braun DR, Bennett ZD, Chanana S, Gus S, Ananiev GE, Guzei IA, Rajski SR, Brunold TC, Bugni TS. J Nat Prod. 2025 Oct. PMID:40998300.
  14. 18-crown-6-ether Utilizes Distinct Allosteric Interactions to Uncouple Transport by the Multidrug Efflux Pump EmrE. Brousseau M, Dash T, Rourke MJ, Kurauskas V, Tonelli M, Hibbs KM, Buller AR, Marty MT, Henzler-Wildman KA. Biochemistry. 2025 Sept. PMID:40899250.
     

Support NMRFAM’s Research:

Your generous contribution helps the work at NMRFAM. Tax-deductible (U.S) can be made through the University of Wisconsin Foundation.

Please make your check payable to: UW Foundation, Account 112152802 and mail to:
Attn: Associate Administrative Program Specialist (Bria Judkins)
University of Wisconsin-Madison
Department of Biochemistry
433 Babcock Dr.
Madison WI 53706

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