e-Newsletter

NMRFAM eNewsletter – April 2024

 

  • NMRFAM will be hosting 2 workshops this summer – Solution NMR Structural Biology (June 4-7) and Solid-state NMR Hands-On (July 23-26). Registration is OPEN!
  • Girolando is a new 900 MHz Bruker US2 magnet at NMRFAM, graciously donated by the University of Illinois at Chicago and installed by Bruker. This month, a new NEO console has been installed, and several solid-state NMR probes are now available for operations, including Phoenix 1.6 mm and 1.2 mm HXY and Black Fox 1.6 and 2.5 mm HCN and other tuning configurations upon request. Please contact us to discuss potential projects that leverage this new high-field spectrometer. 
  • Fleckvieh (900 MHz) is currently configured for solution. Solution users, please submit your requests as soon as possible. 
  • New NAN Data Archive and Data Transport System (NDTS) are Live! In order for your data to be properly archived and transferred, ALL NMRFAM users will need to have an NMRhub account. If you have an NMRbox account, this automatically establishes an NMRhub account and you’re all set. If you do not have an NMRbox/NMRhub account, please create one as soon as possible! Go to https://nmrhub.org/org/ and click the blue Register button in the middle of the page. Email Lai Bergeman your NMRhub username so that we can make sure your data is properly achieved and accessible to you!

 New User/Service Information:

  • NMRFAM provides access to high field (500-900 MHz) solution and solid-state NMR instrumentation and a team of expert staff to assist researchers in experimental design, data acquisition or data analysis. Please contact us with your project ideas and spectrometer needs. Please visit the NMRFAM website for more information.
  • Varian solid-state spectrometers (Ayrshire 750wb, Taurus 600, Ribeye 600) are now producing excellent data, optimized for 1.6 mm and 3.2 mm Phoenix style rotors. We periodically reconfigure Ayrshire among probes including 3.2 mm HCN, 1.6 mm HFCN and 1.6 mm HPCN configurations. Ayrshire has the best 13C sensitivity among the NMRFAM SSNMR spectrometers, whereas Taurus offers the best 1H detection sensitivity. Please consider these enhanced capabilities for your projects and schedule requests.

Solid state NMR spectrometers at NMRFAM:

  • Vosges:  600 MHz Bruker Avance III (TopSpin 3.6) with Bruker 3.2 mm Efree HCN CPMAS, Bruker static and Phoenix 3.2 mm, 1.6 mm and 1.2 mm HFXY probes
  • Taurus:  600 MHz Varian Direct Drive II (Open VnmrJ 3.1) quadruple resonance HFXY console with Varian 1.6 mm T3 HXY and Phoenix 1.6 mm HFXY probes
  • Ribeye:  600 MHz Varian Direct Drive (Open VnmrJ 3.1) triple resonance HXY console with Varian 3.2 mm T3 HXY probe and Phoenix 1.6 mm HXY
  • Ayrshire: 750 MHz wide bore Varian Direct Drive (Open VnmrJ 3.1) quintuple resonance HFXYZ console with several MAS probes including Balun 3.2 mm, BioMAS 3.2 mm, FastMas 1.6 mm HXY and custom-designed 1.6 mm quadruple resonance HFXY and HXYZ, focusing on 19F REDOR.
  • Fleckvieh:  900 MHz Bruker Avance III (TopSpin 3.6) with Phoenix 1.6 mm and 1.2 mm HFXY, Blackfox 1.6 mm HCN/HPC/HC, Bruker 3.2 mm E-FREE HCN and Bruker 1.3 mm HCN probes

Solution state NMR spectrometers at NMRFAM:

  • Kerry:  500 MHz Bruker Avance III with 5 mm TCO HCN cryoprobe and samplejet
  • Kurgan:  600 MHz Bruker Avance III with 5 mm TCI HCN or 1.7mm TCI HCN cryoprobes and samplejet
  • Dexter:  600 MHz Bruker Avance III with 5 mm QCI HFCN cryoprobe or 5 mm QCI HPCN cryoprobe
  • Gelbvieh: 800 MHz Varian VNMRS with 5mm TCI HCN cryoprobe
  • Fleckvieh:  900 MHz Bruker Avance III with 5 mm TCI HCN cryoprobe

 

Donate to NMRFAM:

US deductible donations can be made to NMRFAM. Please write check payable to:

UW Foundation, Account 112152802 and mail to:

Attn: Associate Administrative Program Specialist (Sarah Saunders)

University of Wisconsin-Madison

Department of Biochemistry

433 Babcock Dr.

Madison WI 53706

 

References for recent publications are: 

Predicting multiple conformations via sequence clustering and AlphaFold2.Wayment-Steele HK, Ojoawo A, Otten R, Apitz JM, Pitsawong W, Homberger M, Ovchinnikov S, Colwell L, Kern D. Nature. 2024 Jan. PMID:37956700

 

Functional promiscuity of small multidrug resistance transporters from Staphylococcus aureus, Pseudomonas aeruginosa, and Francisella tularensis.Spreacker PJ, Wegrzynowicz AK, Porter CJ, Beeninga WF, Demas S, Powers EN, Henzler-Wildman KA. Mol Microbiol. 2024 Jan. PMID:38284496

Conformation-dependent lesion bypass of bulky arylamine-dG adducts generated from 2-nitrofluorene in epigenetic sequence contexts. Crisalli AM, Chen YT, Cai A. Li D. Cho BP. Nucleic Acids Res. 2023 Dec. PMID: 37953358

Solution Structure of Poly(UG) RNA. Escobar CA, Petersen RJ, Tonelli M, Fan L, Henzler-Wildman KA, Butcher SE. J Mol. Biol. 2023 Dec. PMID:37924862

Tuning sterol extraction kinetics yields a renal-sparing polyene antifungal. Maji A, Soutar CP, Zhang J, Lewandowska A, Uno BE, Yan S, Shelke Y, Murhade G, Nimerovsky E, Borcik CG, Arango AS, Lange JD, Marin-Toledo JP, Lyu Y, Bailey KL, Roady PJ, Holler JT, Khandelwal A, SantaMaria AM, Sanchez H, Juvvadi PR, Johns G, Hageman MJ, Krise J, Gebremariam T, Youssef EG, Bartizal K, Marr KA, Steinbach WJ, Ibrahim AS, Patterson TF, Wiederhold NP, Andes DR, Pogorelov TV, Schwieters CD, Fan TM, Rienstra CM, Burke MD. Nature. 2023 Nov 8. PMID:37938782. 

13C and 15N Resonance Assignments of Alpha Synuclein Fibrils Amplified from Lewy Body Dementia Tissue. Barclay AM, Dhavale DD, Borcik CG, Milchberg MH, Kotzbauer, Rienstra CM. Biomol NMR Assign. 2023 Dec. PMID:37919529