National Magnetic Resonance Facility at Madison

e-Newsletter

October 2025

  

NEWS & UPDATES

  • Lakenvelder, the 1.1 GHz solid-state NMR (SSNMR) Bruker NEO spectrometer located at NMRFAM, has been fully installed and is available for community access in 2025. We are excited to collaborate with new users and help them leverage this unique resource. To request access, please submit your application through the NAN portal at https://usnan.nmrhub.org/uhf-access-instructions.
  • November 2025 Spectrometer time requests: A friendly reminder that the deadline to reserve spectrometer time for November 2025 in iLab is October 19th at 2 pm CST. If you have questions, please reach out to Lai Bergeman by e-mail or call 608-262-3173.

NEW USER INFORMATION

  • NMRFAM provides access to high and ultra high field (500-1100 MHz) solution and solid-state NMR instrumentation and a team of expert staff to assist researchers in experimental design, data acquisition or data analysis. Please contact us with your project ideas and spectrometer needs. Please visit the NMRFAM website for more information.
  • Solution NMR spectrometers (Fleckvieh 900 MHz, Gelbvieh 800 MHz, Telemark 750 MHz, Kurgan 600 MHz, Dexter 600 MHz, Kerry 500 MHz) are all equipped with 5 mm triple resonance HCN cryoprobes. Additional HPCN and HFCN quad cryoprobes are available at 600 MHz. A 1.7mm cryoprobe is also available at 600 MHz. SampleJet with capacity for up to 500 samples is available at 500 MHz and 600 MHz.
  • Varian SSNMR spectrometers (Ayrshire 750wb, Taurus 600, Ribeye 600) feature a customized version of OpenVNMRJ 3.2 with advanced OPTO automation capabilities. These systems are optimized for 1.6 mm and 3.2 mm Varian and Phoenix probes, including HCN, HFCN, HPCN and other broadband configurations.
  • Bruker SSNMR spectrometers (Lakenvelder 1.1 GHz, Girolando 900 MHz, Vosges 600 MHz) offer unique performance with emphasis on high performance 13C detection at high and ultra-high field.
  • For a full comparison of 13C detection sensitivity among the NMRFAM solid probes, please see Han et al. JMR 2024: https://doi.org/10.1016/j.jmr.2024.107709
  • Our Solution and Solid spectrometer capabilities.

2025 publications:

  1. Selective Detection of a Key Region in Chemotaxis Signaling Protein Complexes by Solid-State NMR. Allen JJ, Wang S, Rienstra CM, Thompson LK. Biophys J. 2025 Sept. PMID:41017145.
  2. 18-crown-6-ether Utilizes Distinct Allosteric Interactions to Uncouple Transport by the Multidrug Efflux Pump EmrE. Brousseau M, Dash T, Rourke MJ, Kurauskas V, Tonelli M, Hibbs KM, Buller AR, Marty MT, Henzler-Wildman KA. Biochemistry. 2025 Sept. PMID:40899250.
  3. The C-terminal Domain of SARS-CoV-2 nsp8 is a Molten Globule in the Absence of Binding Partners. Kurauskas V, Tonelli M, Kirchdoerfer RN, Henzler-Wildman K. J. Mol Biol. 2025 Aug. PMID:40840586
  4. Backbone assignment of a 28.5kDa class A extended spectrum beta-lactamase by high-field, carbon-detected solid-state NMR. Williams CG, Wang S, Thome AF, Warmuth OA, Sakhrani V, Coates L, Rienstra CM, Mueller LJ. Biomol NMR Assign. July 2025. PMID:40716019
  5. Ultrahigh-resolution solid-state NMR for high-molecular weight proteins on GHz-class spectrometers. Wang S, Ravula T, Springer JA, Gor’kov PL, Warmuth OA, William CG, Thome AF, Mueller LJ, Rienstra CM. Sci Adv. July 2025.PMID:40700500
  6. The C-terminus of the multi-drug efflux pump EmrE prevents proton leak by gating transport. Brousseau M, Teng D, Thomas NE, Voth GA, Henzler-Wildman KA. Elife. 2025 July. PMID:40623026.
  7. Probe design for high sensitivity proton-detected solid-state NMR. Borcik CG, Price LE, Heinrich JP, Stringer JA, Rienstra CM. J Magn Reson. 2025 Jun. PMID 40527274.
  8. Alpha-Synuclein Fibril Structures Cluster into Distinct Classes. Milchberg MH, Warmuth OA, Borcik CG, Dhavale DD, Wright ER, Kotzbauer PT, Rienstra CM. J Biophys. 2025 July.
  9. Discovery of New Everninomicin Analogs from a Marine-DerivedMicromonospora sp by Metabolomics and Genomics Approaches. Lee TH, Brittin NJ, Alas I, Roberts CD, Chanana S, Braun DR. Ericksend SS, Guo S. Rajski SR, Bugni TS. Mar Drugs. 2025 Jul. PMID:40863633.
  10. The structure, folding kinetics, and dynamics of long poly(UG) RNA. Petersen RJ, Vivek R, Tonelli M, Roschdi S, Butcher SE. Nucleic Acids Res. 2025 Jul. PMID:40682817.
  11. Structure revision of scytonemim imine and its relationship to scytonemim chromism and cyanobacterial adaptability. Larson GE, Mahmud T, Dergachev ID, Philbin CS Feng Y, de Oliveira CR, Sun HJ, Tucker MJ, Varganov SA, Jeffrey CS. Sci Rep. 2025 Jul. PMID:40664929.
  12. The C-terminus of the multi-drug efflux pump EmrE prevents proton leak by gating transport. Brousseau M, Teng D, Thomas NE, Voth GA, Henzler-Wildman KA. Elife. 2025 Jul. PMID:40623026.
  13. NMR Spectral Alignment Utilizing a CryoEM Motion Correction Algorithm. Hemme CA, Warmuth OA, Wang S, Williams CG, Thome A, Mueller LJ, Rienstra CM, Grant T. bioRxiv (Preprint). 2025 May. PMID:40654982.
  14. Top-Down Scoring of Spectral Fitness by Image Analysis for Protein Structure Validation. Harding BD, DeZonia B, Garg R, Hu Z, Delaglio F, Grant T, Rienstra CM. bioRxiv (Preprint). 2025 May. PMID:40654871.
  15. Selective Detection of a Key Region in Chemotaxis Signaling Protein Complexes by Solid-State NMR. Allen JJ, Wang S, Rienstra CM, Thompson LK, bioRxiv (Preprint) 2025 May. PMID:40463089.
  16. G-quadruplex and i-motif DNA structures form in the promoter of the key innate immune adaptor MYD88. Brown S, Swafford K, McCrury M, Nasrin F, Gragg CQ, Chavan A, Roy Choudhury S, Dickerhoff J, Yang D, Kendrick S. Cell Rep Phys Sci. 2025 May. PMID:40642110.
  17. In situ light-driven pH modulation for NMR studies. Barde A, Han R, Olson MA, Tonelli M, Rienstra CM, Henzler-Wildman K, Ravula T. Angew Chem Int Engl. 2025 March. PMID:40120101.
  18. Removal of Homogeneous Broadening from 1H-Detected Multidimensional Solid-State NMR Spectra. Perras FA, Wang S, Mayer J, Halder M, Paterson AL, Culver DB, Rienstra CM. Anal Chem. 2025 March. PMID 39961603.
  19. High-resolution Cryo-EM Structure Determination of a-Synuclein-A-Prototypical Amyloid Fibril. Sanchez JC, Pierson JA, Borcik CG, Rienstra CM, Wright ER. Bio Protoc. 2025 Feb. PMID: 39959285.
  20. A novel L-RNA aptamer to regulate the pUG fold RNA-induced gene expression in vivo. Liew SW, Cao D, Petersen RJ, Butcher SE, Kennedy SG, Kwok CK. Nucleic Acids Res. 2025 Feb. PMID: 40057375.
  21. Isolation, Structure Elucidation and Biological Evaluation of Lomaiviticins F-H, Dimeric Benzofluorene Glycosides from Marine-Derived Micromonospora sp. Bacterium. Zhang F, Wang W, Braun DR, Ananiev GE, Liao W, Harper MK, Rajski SR, Bugni TS. Mar Drugs. 2025 Feb. PMID: 39997189.
  22. Evolutionary rewiring of the dynamic network underpinning allosteric epistasis in NS1 of the influenza A virus. Gonzales JE 2nd, Kim I, Bastiray A, Hwang W, Cho JH. Proc Natl Acad. Sci. 2025 Feb. PMID: 39977319.
  23. Backbone and Sidechain 1H, 15N and 13C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR. Harding BD, Hiett A, Tonelli M, Wang S, Rienstra CM, Henzler-Wildman KA. Biomol. NMR Assign. Jan 2025. PMID:39829842.
  24. OPTO: Automated Optimization for Solid-State NMR Spectroscopy. Borcik CG, DeZonia B, Ravula T, Harding BD, Garg R, Rienstra CM. J Am Chem Soc. 2025 Jan. PMID:39814553.
  25. Dynamic allostery in the peptide/MHC complex enables TCR neoantigen selectivity. Ma J, Ayres CM, Brambley CA, Chandran SS, Rosales TJ, Perera WWJG, Eldaly B, Murray WT, Corcelli SA, Kovrigin EL, Klebanoff CA, Baker BM. Nat Commun. 2025 Jan. PMID:39833157.

Support NMRFAM’s Research:

Your generous contribution helps the work at NMRFAM. Tax-deductible (U.S) can be made through the University of Wisconsin Foundation.

Please make your check payable to: UW Foundation, Account 112152802 and mail to:
Attn: Associate Administrative Program Specialist (Bria Judkins)
University of Wisconsin-Madison
Department of Biochemistry
433 Babcock Dr.
Madison WI 53706

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