National Magnetic Resonance Facility at Madison

e-Newsletter

September 2024

NEWS & UPDATES

  • Lakenvelder, the Network for Advanced NMR 1.1 GHz solid-state NMR (SSNMR) Bruker NEO spectrometer located at NMRFAM, has been fully installed and is performing at a very high level. We are looking forward to working with new users to leverage this unique resource. Please see more details on the probe capabilities below, and contact us if you have projects that would benefit from 1.1 GHz. Please also stay tuned for announcements with further details and experimental results at https://usnan.nmrhub.org/home
  • Girolando, the 900 MHz Bruker US2 magnet donated by the University of Illinois at Chicago, is now also fully installed. A triple resonance Bruker NEO console supports several SSNMR probes, including Bruker 0.7 mm HCN, Phoenix 1.2 mm and 1.6 mm HXY, and Black Fox 1.6 and 2.5 mm HCN/HPC/HPN, as well as larger (3.2 mm and 5 mm) rotors for materials solids  and several other tuning configurations as required. Please let us know if you have requests for 900 MHz solids!
  • New NAN Data Archive and Data Transport System (NDTS) are Live! In order for your data to be properly archived and transferred, ALL NMRFAM users will need to have an NMRhub account. If you have an NMRbox account, this automatically establishes an NMRhub account and you’re all set. If you do not have an NMRbox/NMRhub account, please create one as soon as possible! Go to https://nmrhub.org/org/ and click the blue Register button in the middle of the page. Email Lai Bergeman your NMRhub username so that we can make sure your data is properly achieved and accessible to you!

NEW USER INFORMATION

  • NMRFAM provides access to high field (500-900 MHz) solution and solid-state NMR instrumentation and a team of expert staff to assist researchers in experimental design, data acquisition or data analysis. Please contact us with your project ideas and spectrometer needs. Please visit the NMRFAM website for more information.
  • Varian SSNMR spectrometers (Ayrshire 750wb, Taurus 600, Ribeye 600) feature a customized version of OpenVNMRJ 3.1 with advanced automation capabilities. These systems are are optimized for 1.6 mm and 3.2 mm Varian and Phoenix probes, including HCN, HFCN, HPCN and other broadband configurations.
  • Bruker SSNMR spectrometers (Lakenvelder 1.1 GHz, Girolando 900 MHz, Vosges 600 MHz) offer unique performance with emphasis on high performance 13C detection at high and ultra-high field.
  • For a full comparison of 13C detection sensitivity among the NMRFAM solids probes, please see our manuscript in press by Han et al. JMR 2024:  https://doi.org/10.1016/j.jmr.2024.107709

SOLID STATE NMR SPECTROMETERS at NMRFAM:

  • Vosges:  600 MHz Bruker Avance III (TopSpin 3.6) with Bruker 3.2 mm E-FREE HCN, Bruker static and Phoenix 1.2 mm, 1.6 mm and 3.2 mm HFXY. Vosges offers flexibility to operate in several modes and is reconfigured among different types of experiments on a regular basis to provide best flexibility for user projects.
  • Taurus:  600 MHz Varian Direct Drive II (Open VnmrJ 3.1) quadruple resonance HFXY console with Varian 1.6 mm T3 HXY and Phoenix 1.6 mm HFXY probes. Taurus is primarily used for 1H-detected 3D and 4D fast magic-angle spinning experiments requiring lengthy periods of signal averaging.
  • Ribeye:  600 MHz Varian Direct Drive (Open VnmrJ 3.1) triple resonance HXY console with Varian 3.2 mm T3 HXY probe and Phoenix 1.2 mm and 1.6 mm HXY. Ribeye is currently being used for several probe and spectrometer console development projects.
  • Ayrshire: 750 MHz wide bore Varian Direct Drive (Open VnmrJ 3.1) quintuple resonance HFXYZ console with several MAS probes including Varian Balun 3.2 mm, BioMAS 3.2 mm, FastMas 1.6 mm HXY, 1.6 mm HFXY and HXYZ, and Phoenix 1.6 mm and 2.5 mm HFXY. This instrument specializes in high sensitivity 13C-detected 3D/4D experiments and 19F REDOR.
  • Girolando:  900 MHz Bruker Avance NEO (TopSpin 4.4) with Bruker 0.7 HCN, Phoenix 1.2 and 1.6 mm HFXY, Blackfox 1.6 and 2.5 mm HCN/HPC/HC.
  • Lakenvelder:  1.1 GHz Bruker Avance NEO (TopSpin 4.4) with Phoenix 1.6 mm and 1.2 mm HFXY, Blackfox 1.6 mm HCN/HPC/HC, Bruker 3.2 mm E-FREE HCN and Bruker 1.3 mm HCN probes.

SOLUTION STATE NMR SPECTROMETERS at NMRFAM:

  •  Kerry:  500 MHz Bruker Avance III with 5 mm TCO HCN cryoprobe and samplejet
  • Kurgan:  600 MHz Bruker Avance III with 5 mm TCI HCN or 1.7mm TCI HCN cryoprobes and samplejet
  • Dexter:  600 MHz Bruker Avance III with 5 mm QCI HFCN cryoprobe or 5 mm QCI HPCN cryoprobe
  • Gelbvieh: 800 MHz Varian VNMRS with 5mm TCI HCN cryoprobe
  • Fleckvieh:  900 MHz Bruker Avance III with 5 mm TCI HCN cryoprobe

Donate to NMRFAM:

US deductible donations can be made to NMRFAM. Please write check payable to:

UW Foundation, Account 112152802 and mail to:
Attn: Associate Administrative Program Specialist (Bria Judkins)
University of Wisconsin-Madison
Department of Biochemistry
433 Babcock Dr.
Madison WI 53706

Recent Publications:

Cross polarization stability in multidimensional NMR spectroscopy of biological solids. Harding BD, Barclay AM, Piehl DW, Hiett A, Warmuth OA, Han R, Henzler-Wildman K, Rienstra CM. J Magn Reson. 2024 Aug. PMID: 38991266

Low power supercycled TPPM decoupling. Garg R, DeZonia B, Paterson AL, Rienstra CM. J Magn Reson. 2024 Aug. PMID: 38991267

Higher Order Structure Differences Among Insulin Crystalline Drugs Revealed by 2D heteronuclear NMR. Wang S, Rienstra CM, Chen K. ChemMedChem. 2024 Aug. PMID:39116305

Solid-State NMR 13C sensitivity at high magnetic field. Han R, Borcik CG, Wang S, Warmuth OA, Geohring K, Mullen C, Incitti M, Stringer JA, Rienstra CM. J Magn Reson. 2024 Aug. PMID: 38991265

Magnetic Susceptibility Modeling of Magic-Angle Spinning Modules for Part Per Billion Scale Field Homogeneity. Schonzart J, Han R, Gennett T, Rienstra CM, Stringer JA. J Magn Reson. 2024 Jul. PMID 38879926

Structure of alpha-synuclein fibrils derived from human Lewy body dementia tissue. Dhavale DD, Barclay AM, Borcik CG, Basore K, Berthold DA, Gordon IR, Liu J, Milchberg MH, O’shea Jy, Rau MJ, Smith Z, Sen S, Summers B, Smith J, Warmuth OA, Perrin RJ, Perimutter JS, Chen Q, Fitzpatrick JAJ, Schwieters CD, Tajkhorshid E, Rienstra CM, Kotzbauer PT. Nat Commun. 2024 Mar. PMID:38553463

Predicting multiple conformations via sequence clustering and AlphaFold2.Wayment-Steele HK, Ojoawo A, Otten R, Apitz JM, Pitsawong W, Homberger M, Ovchinnikov S, Colwell L, Kern D. Nature. 2024 Jan. PMID:37956700

Functional promiscuity of small multidrug resistance transporters from Staphylococcus aureus, Pseudomonas aeruginosa, and Francisella tularensis.Spreacker PJ, Wegrzynowicz AK, Porter CJ, Beeninga WF, Demas S, Powers EN, Henzler-Wildman KA. Mol Microbiol. 2024 Jan. PMID:38284496

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