Workshops

Workshop contents and recordings are available to registered participants at NMRbox.

This workshop covered important considerations in sample preparation and experimental design for protein solution NMR with a focus on ILV-methyl labeled protein experiments. It includes both lectures and hands-on session at the spectrometer for data acquisition and at the computer for data analysis. Presenters include Frank Delaglio, Marco Tonelli, Mike Latham, Art Edison, and Katie Henzler-Wildman.

• Introduction to the Network for Advanced NMR.
• What can NMR do for you? What is NMR good for in the modern era of structural biology?
• Hands-on solution NMR data acquisition of 2D and 3D NMR data for proteins. Focus on experiments studies of U-¹⁵N,²H, ¹H¹³C-methyl-ILV- labeled proteins: ¹H¹⁵N HSQC, ¹H¹⁵N TROSY, ¹H¹³C HMQC, 3D methyl-NOESY-HMQC.
• Data processing, including uniformly- and non-uniformly sampled data with nmrPipe, presented by Frank Delaglio.
• Hands-on structure-based assignment of ILV methyl-labeled proteins using NOESY data and RDCs.
• Introduction to protein methyl dynamics. Practical aspects of data acquisition, processing, and data analysis for MQ-CPMG, methyl ¹H-triple quantum “forbidden” experiments.
• Poster session – share your project and how you are using or planning to use NMR. Network with other participants and get feedback from expert NMR staff.

Solution NMR Structural Biology Workshop 

June 5-9, 2022 at UGA

Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)

Instructors include:

Frank Delaglio (Institute for Bioscience and Biotechnology Research/NIST)

Art Edison, Jim Prestegard, John Glushka, and Alex Eletsky (University of Georgia)

Sam Butcher, Riley Petersen, Marco Tonelli, and Katie Henzler-Wildman (University of Wisconsin-Madison)

Mark Maciejewski (University of Connecticut Health Center)

Solution NMR Structural Biology Workshop – We had a successful Workshop! See you in Georgia next year!
June 20-24, 2022 at NMRFAM
Presented jointly by NMRFAM (UW-Madison) and CCRC NMR Facility (UGA)
Presenters include Katie Henzler-Wildman, Marco Tonelli, Vilius Kurauskas (NMRFAM) and Art Edison, Jim Prestegard, Alex Eletsky (CCRC), and Jeff Hoch (UConn)

2022 Solution NMR Topics

Virtual Workshop – Access Anytime!
Presented June 2021
Step by step training in how to assign your protein using NMRFAM-Sparky

Manual Backbone Assignments – Szymon Ciesielski
Q&A video 1
Q&A video 2

Manual Sidechain Assignments – Marco Tonelli
Q&A video 1
Q&A video 2
Updated side chain assignment folder

APES(Automated Peak Picking of Spectra) – Mehdi Rahimi
Q&A video

I-PINE – Kumaran Baskaran
Q&A video

Virtual Workshop – Access Anytime!
Presented June 2020
Step by step training in how to assign your protein using NMRFAM-Sparky

NMRFAM Protein Structure Determination Workshop – Milo Westler

NMRFAM SPARKY – Woonghee Lee

Manual Backbone Assignments – Szymon Ciesielski

Manual Sidechain Assignments – Marco Tonelli

APES(Automated Peak Picking of Spectra)  – Mehdi Rahimi

I-PINE  – Kumaran Baskaran

BMRB Overview and data deposition  – Kumaran Baskaran