University of Wisconsin–Madison
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Overview

NMRFAM Overview


The National Magnetic Resonance Facility at Madison (NMRFAM) is a state of the art NMR spectrometer facility located in the Department of Biochemistry at the University of Wisconsin-Madison. NMRFAM equipment and resources are available to any scientist worldwide. Our experienced staff is available to train users as well as provide consultation and collaboration on experimental design, data collection, and analysis. NMRFAM is funded through NIGMS Biomedical Technology Research Centers (BTRC) and from user fees.

Technology Research and Development (TR&D)

1 – Fast data collection and automated data analysis of biomacromolecules
2 – Technology for larger proteins, nucleic acids, and complexes
3 – Investigations of metal-containing (paramagnetic) proteins
4 – Dynamics of macromolecules
5 – Structure-function investigation of RNA molecules and their complexes with metal ions and proteins
6 – Metabolomics and natural products

Service and Collaborations

1 – Low-cost access to NMR instrumentation which includes a mixture of two Agilent and seven Bruker spectrometers:  500(2), 600(4), 750, 800, 900 MHz
2 – HPLC-Solid Phase Extraction-liquid handling for 1.7 and 5 mm NMR tubes with integrated MicrOTOF mass spectrometer
3 – Bruker AXS Nanostar SAXS (small angle X-ray scattering)
4 – External access to processing and analysis servers, metabolomics database (LINK), software packages, and pulse program libraries
5 – Consultation with current and potential users

Training

1 – Hands-on training for facility users
2 – Annual workshops offered
3 – Development of new software tools

Dissemination

1 – Pulse programs and software tools
2 – Newsletter and website
3 – Publications, lectures, posters, workshops, and personnel exchanges
4 – Collaborations with other facilities in the US and abroad