• Overview

NMRFAM Overview

The National Magnetic Resonance Facility at Madison (NMRFAM) is a state of the art NMR spectrometer facility located in the Department of Biochemistry at the University of Wisconsin-Madison. NMRFAM equipment and resources are available to any scientist worldwide. Our experienced staff is available to train users as well as provide consultation and collaboration on experimental design, data collection, and analysis. NMRFAM is funded through NIGMS Biomedical Technology Research Centers (BTRC) and from user fees.

Technology Research and Development (TR&D)

1 - Fast data collection and automated data analysis of biomacromolecules
2 - Technology for larger proteins, nucleic acids, and complexes
3 - Investigations of metal-containing (paramagnetic) proteins
4 - Dynamics of macromolecules
5 - Structure-function investigation of RNA molecules and their complexes with metal ions and proteins
6 - Metabolomics and natural products

Service and Collaborations

1 - Low-cost access to NMR instrumentation which includes a mixture of two Agilent and seven Bruker spectrometers:  500(2), 600(4), 750, 800, 900 MHz 
2 - HPLC-Solid Phase Extraction-liquid handling for 1.7 and 5 mm NMR tubes with integrated MicrOTOF mass spectrometer
3 - Bruker AXS Nanostar SAXS (small angle X-ray scattering)
4 - External access to processing and analysis servers, metabolomics database (LINK), software packages, and pulse program libraries
5 - Consultation with current and potential users


1 - Hands-on training for facility users
2 - Annual workshops offered
3 - Development of new software tools


1 - Pulse programs and software tools
2 - Newsletter and website
3 - Publications, lectures, posters, workshops, and personnel exchanges
4 - Collaborations with other facilities in the US and abroad

Become an NMRFAM User
Contacts | Map | Policy
Instrumentation w/Rates
Pulse Programs
Remote Access to NMRFAM
Spectrometer Live Calendar
Sundial (Request NMR Time)
Software Tools
e-News Sign-Up

Biochemistry 800 - Practical Nuclear Magnetic Resonance Theory 

Biochemistry 801 - Biochemical Applications of Nuclear Magnetic Resonance 

"The Future of NMR-Based Metabolomics, Current Opinion in Biotechnology (2017), pp. 34-40

Documents on the use of the Bruker-Axs Nanostar,SAXS instrument and analysis of SAXS data are now available.

NMRFAM-SPARKY Distribution - the popular NMR analysis program SPARKY recompiled (including updated python and Tcl/Tk) with incorporation of PINE-Sparky, enhancements to import/export to the structural analysis program CYANA, and other useful python extensions.

ADAPT-NMR Enhancer: Complete Package for Reduced Dimensionality in Protein NMR Spectroscopy

RNA-PAIRS: RNA Probabilistic Assignment of Imino Resonance Shifts

PACSY, a Relational Database Management System for Protein Structure and Chemical Shift Analysis 


Donate to NMRFAM. US tax-deductible donation can be made to NMRFAM
Please write check payable to "UW Foundation, Account 112152802"  
And mail to: 
Attn: Sarah Lynn Traver Saunders
Associate Administrative Program Specialist 
University of Wisconsin-Madison 
433 Babcock Drive 
Madison, WI 53706 
Tel: 608-265-2507 or email 


1st: Lai Bergeman 
Rm 171; Phone 262-3173

2nd: Milo Westler
Rm B160; Phone 263-9599

3rd: Paulo F. Cobra
Rm B224; Phone 265-3303

4th: Marco Tonelli
Rm B160; Phone 263-9493

5th: John Markley
Rm 171A; Phone 263-9349

We welcome your questions and feedback!

NMRFAM Established 1987