University of Wisconsin–Madison

NMRFAM Protein Labeling Strategies

Uniform 15N and/or 13N

Uniform 2H, 15N, and 13C

Amino-acid selective

Dual labeling of adjacent residues

Methyl labeling

Stereo array isotopic labeling (SAIL)

CESG Technology Dissemination Reports (Protein Production Protocols)

More Protein Production Protocols

These are target specific and may involve contributions from other PSI centers:

Hint: Choose for example under Experimental:

Protocol Used: NMR


Reported Progress: HSQC Satisfactory or NMR Structure


Trial Stop Status: Structure Successful