NMRFAM Courses and News

Fall Semester

PDB ID: 1G90

Biochem 800 – Practical Nuclear Magnetic Resonance Theory. Every fall semester, Dr. Milo Westler, Director of NMRFAM, teaches Biochem 800 – Practical Nuclear Magnetic Resonance Theory (2 credits) at UW-Madison on Tuesdays and Thursdays at 11:00am in Room 2221 in the DeLuca Biochemical Sciences Building. Scope of the course includes study of multipulsed, multinuclear, and multidimensional NMR spectroscopy, emphasis on the vector and product, and operator formalisms for the analysis of modern pulse sequences. Course format is lecture and discussion and grades will be based on participation and problem sets. Topics to be covered: The Vector Paradigm; Coherence and Magnetization; Radio Frequency (Rf) Excitation; Chemical Shifts; Signal Detection; Fourier Transformation; Coherence Transfer Pathways and Phase Cycling; Pulsed Field Gradients; Scalar Coupling; Product Operator Formalism; Coherence Transfer; Isotope Directed Editing; Two-Dimensional NMR; Multi (>2)-Dimensional NMR; 1H, 13C, and 15N Triple Resonance 3D and 4D NMR; Pulse Concatenation and Sequence Optimization; Relaxation. Prerequisites are introductory NMR (e.g., organic chemistry), basic trigonometry, elementary physics, and physical chemistry.

PDB ID: 1G90

Spring Semester

Biochem 801 – Biochemical Applications of Nuclear Magnetic Resonance. Every spring semester, Professor John Markley and a team of scientists from NMRFAM teach Biochem 801 - Biochemical Applications of Nuclear Magnetic Resonance (2 credits) This course consists of lectures surveying current solution-state nuclear magnetic resonance techniques used in biochemical research; the emphasis is on how data are acquired and on practical applications. Prerequisite: Consent of instructor. Sample Syllabus.


18 October 2016

Integrative NMR for biomolecular research. NMR spectroscopy is a powerful technique for determining structural and functional features of biomolecules in physiological solution as well as for observing their intermolecular interactions in real-time. However, complex steps associated with its practice have made the approach daunting for non-specialists. We introduce an NMR platform that makes biomolecular NMR spectroscopy much more accessible by integrating tools, databases, web services, and video tutorials that can be launched by simple installation of NMRFAM software packages or using a cross-platform virtual machine that can be run on any standard laptop or desktop computer. The software package can be downloaded freely from the NMRFAM software download page (http://pine.nmrfam.wisc.edu/download_packages.html), and detailed instructions are available from the Integrative NMR Video Tutorial page (http://pine.nmrfam.wisc.edu/integrative.html). Continue reading at PubMed.


21 November 2014

Bruker Nanostar

A small angle X-ray scattering (SAXS) instrument, Bruker-AXS Nanostar, have been installed and is operational. A Bruker-Axs Nanostar with a rotating anode source is now installed (funding obtained from NIH-shared instrumentation, Professor Sam Butcher, PI, UW-Madison Department of Biochemistry). The system resides in NMRFAM and is available to users of the facility. More information about the system is at Bruker's website

Several documents on the use of the instrument and SAXS data analysis are available.


21 November 2014

NMRFAM-SPARKY is a recently updated version of SPARKY (https://www.cgl.ucsf.edu/home/sparky) that supports user-defined enhancements. We have used these capabilities to develop new tools in support of packages generated at NMRFAM for automated protein assignment and structure determination. The added features support:

(1) interfacing with servers offering new technologies,
(2) tools for data visualization and verification, and
(3) new protocols for maximizing the efficiency of NMR data analysis. In NMRFAM-SPARKY, we have refined these SPARKY enhancements through their use in our annual workshops by participants with varying experience in protein NMR spectroscopy.

15 February 2013

ADAPT-NMR EnhancerADAPT-NMR Enhancer: Complete Package for Reduced Dimensionality in Protein NMR Spectroscopy. ADAPT-nuclear magnetic resonance (ADAPT-NMR) offers an automated approach to the concurrent acquisition and processing of protein NMR data with the goal of complete backbone and side chain assignments. What the approach lacks is a useful graphical interface for reviewing results and for searching for missing peaks that may have prevented assignments or led to incorrect assignments. Because most of the data ADAPT-NMR collects are 2D tilted planes used to find peaks in 3D spectra, it would be helpful to have a tool that reconstructs the 3D spectra. Continue reading at PubMed

1 October 2012

PACSYPACSY, a Relational Database Management System for Protein Structure and Chemical Shift Analysis. PACSY (Protein structure And Chemical Shift NMR spectroscopy) is a relational database management system that integrates information from the Protein Data Bank, the Biological Magnetic Resonance Data Bank, and the Structural Classification of Proteins database. PACSY provides three-dimensional coordinates and chemical shifts of atoms along with derived information such as torsion angles, solvent accessible surface areas, and hydrophobicity scales. Continue reading at PubMed

1 April 2012

RNA-PAIRSRNA-PAIRS: RNA Probabilistic Assignment of Imino Resonance Shifts. The significant biological role of RNA has further highlighted the need for improving the accuracy, efficiency, and the reach of methods for investigating RNA structure and function. Nuclear magnetic resonance (NMR) spectroscopy is vital to furthering the goals of RNA structural biology because of its distinctive capabilities. However, the dispersion pattern in the NMR spectra of RNA makes automated resonance assignment, a key step in NMR investigation of biomolecules, remarkably challenging. Continue reading at PubMed

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Biochemistry 800 - Practical Nuclear Magnetic Resonance Theory 

Biochemistry 801 - Biochemical Applications of Nuclear Magnetic Resonance 

"The Future of NMR-Based Metabolomics, Current Opinion in Biotechnology (2017), pp. 34-40

Documents on the use of the Bruker-Axs Nanostar,SAXS instrument and analysis of SAXS data are now available.

NMRFAM-SPARKY Distribution - the popular NMR analysis program SPARKY recompiled (including updated python and Tcl/Tk) with incorporation of PINE-Sparky, enhancements to import/export to the structural analysis program CYANA, and other useful python extensions.

ADAPT-NMR Enhancer: Complete Package for Reduced Dimensionality in Protein NMR Spectroscopy

RNA-PAIRS: RNA Probabilistic Assignment of Imino Resonance Shifts

PACSY, a Relational Database Management System for Protein Structure and Chemical Shift Analysis 


Donate to NMRFAM. US tax-deductible donation can be made to NMRFAM
Please write check payable to "UW Foundation, Account 112152802"  
And mail to: 
Attn: Sarah Lynn Traver Saunders
Associate Administrative Program Specialist 
University of Wisconsin-Madison 
433 Babcock Drive 
Madison, WI 53706 
Tel: 608-265-2507 or email 


1st: Lai Bergeman 
Rm 171; Phone 262-3173

2nd: Milo Westler
Rm B160; Phone 263-9599

3rd: Paulo F. Cobra
Rm B224; Phone 265-3303

4th: Marco Tonelli
Rm B160; Phone 263-9493

5th: John Markley
Rm 171A; Phone 263-9349

We welcome your questions and feedback!

NMRFAM Established 1987