The Center for Eukaryotic Structural Genomics (CESG) was a specialized research center supported by the Protein Structure Initiative (PSI) of the National Institute of General Medical Sciences (NIGMS), one of the National Institutes of Health (NIH) from 2001-2010. CESG was housed within the Department of Biochemistry at the University of Wisconsin-Madison (Madison, WI) and also the Department of Biochemistry at the Medical College of Wisconsin (Milwaukee, WI). CESG developed new methods and technologies to address unique eukaryotic bottlenecks and disseminated its methodologies and experimental results to the scientific community worldwide.
Here’s a list of technology dissemination reports:
- Sesame Laboratory Information Management for Structural Genomics
- Target Trace and PepcDB Generation Software
- Use of a High-Resolution Genome Tiling Array to Identify the Presence of Target Genes in a cDNA Pool
- Evaluation of Low-Complexity in Amino Acid Sequences for Target Selection in Structural Genomics
- Economical Approaches to the Production of [Se-Met]-, [U-15N- and [U13C, U15N]-Labeled Proteins from Escherichia Coli Cells
- Factorial Evolved Auto-Induction Medium
- Wheat Germ Cell-Free Protein Production and Stable-Isotope Labeling Platform for NMR-Based Structural Proteomics
- Small-Scale Semi-Automated Purification of Eukaryotic Proteins for Structure Determination
- Ensemble Refinements of Protein Structures
- ACMI-Automatic Crystallography Map Interpretation
- HIFI-NMR (High-Resolution Iterative Frequency Identification for NMR)
- HIFI-C (High-resolution Iterative Frequency Identification of Couplings)
- LACS (Linear Analysis of Chemical Shifts)
- PINE (Probabilistic Inference Network of Evidence)
- Structural Genomics Methods Applied to Production of TEV Protease
- Structural Genomics Methods Applied to Production of the Monotopic Membrane Protein Human Cytochrome b5 and In Situ Delivery to Liposomes
- Customized Expression Vector Platform
- Materials Distribution
- CrystalFarm Pro Software Package
- Activity and Topology Determination of In Vitro Expressed Membrane Proteins
- Co-Expression of Protein:Protein Complexes Using a Wheat Germ Cell-Free System
- Development of Detergent Arrays for Membrane Protein Compatibility Screening
- Expression and Purification of Bacteriorhodopsin using Protemist XE with Detergents
- Protease Based Salvage Pathways
- Protease Based Salvage Pathways: Screening Prior to Large Volume Cleavage
- New Expression Vectors for Small-Scale Cell-Based Protein Production and Purification
- Platform Expression Vectors in Use at CESG
- Identification of Protein-Protein Complex Formation and Co-Expression in Cell-Based Platform
- Detergent Solubilization Screening for Membrane Proteins Expressed in E. coli
- Cell-Free Expression Screening Through Overlap Extension PCR
- Optimization of Membrane Protein Production in E. coli and Robotic Purification