Macromolecular Complexes


 

 

The need of preparing multiple isotope cross-labeled samples for NOE based structural studies of protein-protein or protein-DNA(or RNA) complexes can be circumvented by measuring residual dipolar couplings (RDCs) and using ambiguous interfacial distance constraints.

 

RDCs provide relative orientational constrains for docking molecules in a complex. The method can be extended to proteins with multiple ligands or even multimeric protein-ligand complexes, by exploiting the symmetry properties of the macromolecules involved.

 

NMRFAM can assist users in:

  • preparing samples in various aligning media, including liquid crystals (LC) and stretched acrylamide gels (SAG)
  • measuring highly precise RDCs using TROSY based pulse sequences (NH and CH ARTSY pulse sequences for protonated and perdeuterated proteins and RNA/DNA are implemented on our  Bruker and Agilent spectrometers)
  • measuring and using Residual Chemical Shift Anisotropy (RCSA) constraints
  • processing and analyzing spectra to extract RDC or RCSA constraints
  • measuring SAXS data
  • performing complex structure elucidation using XPLOR-NIH

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"The Future of NMR-Based Metabolomics, Current Opinion in Biotechnology (2017), pp. 34-40

Telluride School on Biomolecular Structure and Dynamics;Theory and Experiment (July 24-31, 2017) 

AMPERE NMR School (June 25th - July 1, 2017: Zakopane, Poland)

Documents on the use of the Bruker-Axs Nanostar,SAXS instrument and analysis of SAXS data are now available.

NMRFAM-SPARKY Distribution - the popular NMR analysis program SPARKY recompiled (including updated python and Tcl/Tk) with incorporation of PINE-Sparky, enhancements to import/export to the structural analysis program CYANA, and other useful python extensions.

ADAPT-NMR Enhancer: Complete Package for Reduced Dimensionality in Protein NMR Spectroscopy

RNA-PAIRS: RNA Probabilistic Assignment of Imino Resonance Shifts

PACSY, a Relational Database Management System for Protein Structure and Chemical Shift Analysis 

 

Donate to NMRFAM. US tax-deductible donation can be made to NMRFAM
Please write check payable to "UW Foundation, Account 112152802"  
And mail to: 
Attn: Sarah Lynn Traver Saunders
Associate Administrative Program Specialist 
University of Wisconsin-Madison 
433 Babcock Drive 
Madison, WI 53706 
Tel: 608-265-2507 or email 

 

1st: Lai Bergeman 
Rm 171; Phone 262-3173

2nd: Milo Westler
Rm B160; Phone 263-9599

3rd: Mark Anderson
Rm B224; Phone 265-3303

4th: Marco Tonelli
Rm B160; Phone 263-9493

5th: John Markley
Rm 171A; Phone 263-9349

We welcome your questions and feedback!

NMRFAM Established 1987