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Salt Tolerant Susceptibility Matched Slot Tubes

slot_tube.png 

This special Shigemi slot tube provides the highest sensitivity and shortest 90º pulse with any probe when working with high salt NMR samples.

The tube has a  susceptibility matched glass cavity that keeps solution away from hot spots (which contribute to S/N loss and heating). It consists of an outer tube and an insert (plunger) similar to those used in regular Shigemi symmetrical tubes. The outer tube has an outside diameter of 5.0 mm and a sample cavity with a magnetic susceptibility matched glass bottom. The cavity is 2mm wide and 20 mm high; its cross-section has straight sides and arc shape ends looking almost rectangular.

 

Note Well: Do not to mix the inserts (plungers) between regular Shigemi symmetrical tubes and these special slot tubes, since their magnetic susceptibility matched glass is different.

Benefits:

  • Smaller sample volume (~160µL)
  • RF sample heating is minimized (important for high salt samples)
  • Higher S/N per unit volume 
  • Overall S/N is 35% higher than that of a conventional 5 mm Shigemi microcell tube (280µL) in the presence of 300 mM NaCl


Requirements:
NMRFAM has the positioning tools needed to orient the salt tolerant tubes in both Varian and Bruker probes.

Varian:

positioning_tool_slot_tube.png 

positioning tool in 900MHz spectrometer 

Bruker's Shaped or Shigemi Salt Tolerant Tube Shuttles

NMRFAM has special Bruker shuttles (spinners) for both the round Shigemi slot tube and for the rectangular shaped salt tolerant Bruker tubes.

shaped_shigemi_tube_shuttle.png 

 

More info:

Shigemi's salt tolerant slot tube web page

Research paper about the slot tubes

 

NMRFAM High Salt Data:

signal_to_noise_high_salt1.png

 

 

 signal_to_noise_high_salt2.png

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Biochem 800 – Practical Nuclear Magnetic Resonance Theory

Biochem 801 – Biochemical Applications of Nuclear Magnetic Resonance

"The Future of NMR-Based Metabolomics, Current Opinion in Biotechnology (2017), pp. 34-40

Telluride School on Biomolecular Structure and Dynamics;Theory and Experiment (July 24-31, 2017) 

AMPERE NMR School (June 25th - July 1, 2017: Zakopane, Poland)

Documents on the use of the Bruker-Axs Nanostar,SAXS instrument and analysis of SAXS data are now available.

NMRFAM-SPARKY Distribution - the popular NMR analysis program SPARKY recompiled (including updated python and Tcl/Tk) with incorporation of PINE-Sparky, enhancements to import/export to the structural analysis program CYANA, and other useful python extensions.

ADAPT-NMR Enhancer: Complete Package for Reduced Dimensionality in Protein NMR Spectroscopy

RNA-PAIRS: RNA Probabilistic Assignment of Imino Resonance Shifts

PACSY, a Relational Database Management System for Protein Structure and Chemical Shift Analysis 

 

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Please write check payable to "UW Foundation, Account 112152802"  
And mail to: 
Attn: Sarah Lynn Traver Saunders
Associate Administrative Program Specialist 
University of Wisconsin-Madison 
433 Babcock Drive 
Madison, WI 53706 
Tel: 608-265-2507 or email 

 

1st: Lai Bergeman 
Rm 171; Phone 262-3173

2nd: Milo Westler
Rm B160; Phone 263-9599

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Rm B224; Phone 265-3303

4th: Marco Tonelli
Rm B160; Phone 263-9493

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Rm 171A; Phone 263-9349

We welcome your questions and feedback!

NMRFAM Established 1987